AML - FMS-Like Tyrosine (FLT3) Gene Mutation Test Cost & Procedure

An internal tandem duplication mutation is the mutant type that affects FLT3 most frequently. This type of mutation causes the DNA from exons 14 or 15 to be duplicated. Therefore, FLT3 exhibits or acts as a poor prognostic predictor in various circumstances or outcomes. The codon for an aspartate residue is located in FLT3's activation loop. Most studies show that both types of FLT3 mutations cause FLT3 to become activated.

Preparation for FLT3 mutations and Acute Myeloid Leukemia Test:

So it makes sense—and is fairly obvious—to see a doctor before the test. Talk to your doctor about it even if you think no preparations are required to be on the safe side and to prevent mistakes during the test. The doctor may ask whether you have any current allergies and what kind of medication you are taking. Because the lab test may contain reagents or components that could cause your allergies, you must be upfront and sincere with the doctor.

Test for FLT3 mutations in Acute Myeloid Leukemia: Uses:

• The test is helpful for those with acute myeloid leukemia. The fact that the test serves as a prognostic indicator during the process is one crucial benefit that outweighs all other benefits.
• It displays which of the two mutational forms was detected, whether one or both were, or neither was.
•  It is known as capillary electrophoresis in some labs. The test looks for two different types of mutations, namely Asp835, a position in the codon, and the coding sequence going through internal tandem duplication to create the intracellular juxtamembrane domain.

Procedure:

• A sample is collected.
• The genomic DNA from the sample's currently nucleated cells is then extracted.
• PCR is carried out by using two sets of DNA-derived primers.