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A test to measure the activity of amylase in bodily fluids. Clinical laboratories frequently use the measurement of -amylase activity in serum, urine, or other body fluids for the diagnosis of pancreatic diseases. The properties of various laboratory techniques for the enzyme assay are explained. According to a survey of clinical laboratories, 35% used procedures employing dye-labeled starches, and 30% used amyloclastic methods to do their assays. The test of amylase activity in serum and urine is covered in this chapter
A straightforward technique depends on the release of soluble fragments with sizes distinct from the substrate and uses dye-marked (dye-coupled) starch as the substrate for amylase. Such substrates, such as Reactone-red-2B-amylopectin, Blue starch polymer 51-A, Remazol Brilliant Blue Starch, Cibachron Blue F-3GA Amylose, and Procion Brilliant Red M-2BS-amylopectin, are insoluble in the reaction mixture. Following substrate digestion, the dye concentration in the supernatant is photometrically determined. Small soluble fragments that have been liberated from the substrate and are combined with dye are what are being measured.
Amylase is a protein or enzyme that facilitates digestion. A greater or lower value could point to a pancreatic issue, an infection, alcoholism, or another illness.
An amylase body fluid test can be requested in addition to, or just after, an amylase blood test. Amylase in the fluid can help in the diagnosis of illnesses of the pancreas and salivary glands.
This test can be necessary if your doctor believes your pancreas isn't functioning properly. The following are signs of pancreatic enlargement (pancreatitis):
You might be asked by your doctor to collect all of your pee on one day. Several samples taken throughout the course of the day might give a more accurate picture. You will receive a container from the lab along with thorough instructions for gathering samples at home.
Your examination outcomes may be insufficient than anticipated if you have undue cholesterol, triglycerides, and pancreatic disease.
Urine tests can have their results impacted by other bodily fluids, particularly saliva. In comparison to urine, saliva has 700 times more amylase.
Test Type | Amylase Pancreatic Alpha Body Fluid |
Includes | Amylase Pancreatic Alpha Body Fluid (Pathology Test) |
Preparation | |
Reporting | Within 24 hours* |
Test Price |
₹ 480
|
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