BCR-ABL Quantitative PCR with BPA (MRD) Test Cost & Procedure

A crucial tool for directing tyrosine kinase inhibitor therapy and the long-term follow-up of patients with chronic myeloid leukaemia is molecular response monitoring (MR), which is done using quantitative polymerase chain reaction (PCR) for BCR-ABL1 (CML). The International Scale (IS) is used to standardise the results of MR monitoring, and precise time-dependent molecular milestones have been incorporated into therapy recommendations to define an optimal response and treatment failure. It has been questioned whether it is appropriate to monitor the MR monitoring in CML using peripheral blood (PB) rather than bone marrow aspirate, as is customary.

BCR-ABL

The BCR-ABL1 fusion gene (BCR, BCR activator of RhoGEF and GTPase, NG 009244.2; ABL1, ABL proto-oncogene 1, nonreceptor tyrosine kinase, NG 012034.1) is a hallmark of chronic myeloid leukaemia (CML), a myeloproliferative neoplasm. Reverse transcriptase polymerase chain reaction (RT-PCR) or fluorescence in situ hybridization (FISH) can be used to diagnose the reciprocal translocation t(9;22)(q34;q11) that is linked to the BCR-ABL1 fusion [1]. Tyrosine kinase inhibitors that target BCR-ABL1 are extremely efficient. They have been adopted as the gold standard of care for CML patients [2]. Quantitative RT-PCR (qRT-PCR) monitoring of molecular response (MR) is frequently employed because it offers critical prognostic data, particularly for patients receiving TKI treatment. In actuality, time-dependent molecular milestones for the definition of a successful outcome and a failed treatment.

Prerequisites for Specimens-Whole blood, bone marrow, or cell pellet specimen Volume 1 to 2 mL bone marrow or 3 to 5 mL whole blood Lowest Volume 3 mL of whole blood or 1 mL of bone marrow

Container/ Green-topped tubes for sodium heparin, yellow-topped tubes for ACD-A, tan-topped tubes for K2-EDTA, or pink-topped tubes for K2-EDTA

Collection- submit with no special requirements.

specimens must be delivered to the lab within 24 hours. In the test request form, indicate the collection date and time.

Storage Guidelines- submit with no special requirements. Refrigerate at 2°C to 8°C if the specimen needs to be kept for longer than 48 hours.

Rejection Reasons- Specimen doesn't fit the requirements for collection; frozen bone marrow or entire blood; leaky tube.