Cholera is a bacterial infection caused by the bacterium Vibrio cholerae. These bacteria live in the small intestine through contaminated water and consumption of food due to a lack of social awareness and development which...
Cholera is a bacterial infection caused by the bacterium Vibrio cholerae. These bacteria live in the small intestine through contaminated water and consumption of food due to a lack of social awareness and development which indicates the threat to public health. Cholera is an acute alarming and life-threatening disease that causes many symptoms related to your digestion such as watery diarrhea, dehydration or kill within an hour if it is not treated. This infection can transmitted through food and water. Their sign and symptoms can be acute and severe.
History behind cholera: During the 19th century, cholera spread worldwide. Pandemics killed millions of people over all landmasses. The current (seventh) widespread began in South Asia in 1961, reached Africa in 1971, and the Americas in 1991. Cholera is presently endemic in numerous nations.
What are the causes of Cholera?
As I mentioned above cholera is caused by a bacterial infection that is infected by feces and found in food or water. There are many sources including:
- Water supplies by Municipal.
- Local purchasing of any food and drink.
- Uncooked or Raw seafood caught from polluted water (Sewage).
- Open street food.
What are the Cholera Symptoms?
- Dehydration
- Fast heart rate
- Thirst
- Muscle cramps
- Dry mucous membranes
- Stomach ache
Cholera Treatment and Prevention
- Drinking pure water.
- Washing before using the fruits & vegetable
- Proper washing of the utensils.
- Vaccination for cholera.
- Clean your surroundings.
Method for Testing
PCR techniques are a rapid, highly specific, and sensitive method for Cholera PCR detection in a patient’s sample. In PCR, specific primers and fluorescent probes are synthesized for the Cholera and amplified so that a very small number of genes of interest can also get detected by the detector, and the result can be recorded and visualized.
Procedure for Cholera PCR test
Sample Collection: Blood Sample drawn in EDTA Tube from the vein of the patient’s arm.
Sample Transport: Collected EDTA blood tube stored at 2 to 4OC temperature and transported to testing molecular Lab.
Sample Processing: DNA is isolated from Blood samples using fully automated Nucleic acid extraction machines. After that Cholera PCR is amplified with the help of a PCR machine.
Result Analysis: The doctor will analyze the result, whether the Cholera is amplified or not, which concludes the positive or Negative result for Cholera. Finally, the Report is authenticated and released.
Requirement for Cholera PCR Testing
There is no special requirement for testing however it’s better to inform the phlebotomist if you are taking any medication or have any allergy or medical condition such as bleeding or clotting.
The risk associated with Cholera Testing
There is no risk involved in Cholera testing; therefore the patient does not require any special attention after testing.
Note for patients: although blood is collected from experienced phlebotomists, there are chances that patients may feel discomfort or pain at the vein puncture site which is normal.
Cholera PCR test Cost in Delhi /NCR
The price of the Cholera PCR test varies from lab to lab and depends on Lab certification; the instrument used, and qualified technical staff and doctors.
Cholera PCR test at Ganesh Diagnostic and Imaging Centre
Ganesh Diagnostic and Imaging Center is a National Accreditation Board for Testing and Calibration Laboratories (NABL) certified lab for Cholera testing. NABL accreditation reflects the competency of laboratory infrastructure, technical staff, and equipment based on national and international standards.
The lab has state-of-the-art machines such as a Bio-Rad Real-Time PCR machine, fully closed Truelab Quattro Real-Time Quantitative PCR Machine, Genetix HT 96 Purifier Machine, and qualified techniques to perform testing and doctor for result analysis and report authentication.
The use of fully automated machines at Ganesh Diagnostic eliminates the errors that can happen due to human intervention. All this makes sure that the patient will receive a reliable and 100% accurate diagnostic report for the test.
The report for the Cholera PCR test is provided within 24 hr of sample collection.
Free Home collection and Online report facilities are provided by Ganesh Diagnostic so that patients can get tests from the comfort of their homes.
The facility for Pathology testing operates 24x7, 365 days, providing service to patients round the clock!
At Ganesh Diagnostic and Imaging Centre, all diagnostic test prices are very reasonable, and currently Cholera PCR test is done at 50% OFF.
