Chromosome 17P13 Deletion Test Cost & Procedure

Chromosome 17p13: what is it? According to whether a duplication or elimination of the portion has taken spot the area of chromosome 17p13.3 is a part of genomic instability that is connected to a variety of rare neurodevelopmental genetic ailments. Microdeletions on the 17p13 chromosome.

Test F P53 Code

synonyms for p53, 17p13-, and del(17) (p13,1)

Associations

Myeloma, B-cell lymphoproliferative disorder (LPD), chronic lymphocytic leukaemia (CLL), and B-cell prolymphocytic leukaemia (PLL)

Fluorescence in situ hybridization is the procedure accomplished (FISH)

Time to complete: 3-5 days

Prerequisites for Specimens

5 mL of peripheral blood in heparin sodium

3mL of sodium heparin-infused bone marrow

Fixed cytogenetically prepared cells with pellet visible in 3:1, methanol:acetic acid in sterile centrifuge tube

Variety Stability

Blood and bone marrow are stable for up to 72 hours at temperatures between 4°C and 25°C.

Fixed cell pellets can be kept for years if they are kept between -28°C and 15°C.

Keeping and Handling

During transit, temperatures range from 4 to 25 degrees, however samples can be shipped in chilled gel packs. Keep the gel pack away from the specimen at all times. Avoid freezing. Avoiding extreme temperatures is advised.

Rejection Reasons

Sample with bleeding; specimen that has experienced a high temperature; anticoagulant that is harmful to cells; There are not enough cells.

About Range

Two orange and two green signals are seen in a typical cell. The signal pattern of one orange and two green indicates an aberrant cell.

Description

One of the most often altered genes in human cancer is the p53 tumour suppressor gene; deletions and mutations have been found in a variety of solid tumours and haematological illnesses. 60–80% of cases of Hodgkin disease, 30% of adult T-cell leukaemia, 30% of high grade B-cell NHL (rare in low grade NHL), 20–30% of blast crisis CML cases, 5% of MDS, 15% of AML, 15% of CLL, 5%–10% of multiple myelomas, and 2% of ALL have been shown to contain p53 gene mutations. Hematological cancers that have p53 gene mutations typically have a bad prognosis.

The Li-Fraumeni syndrome, a hereditary cancer-prone condition frequently characterised by more than one primary tumour with cancers frequently appearing at a younger age than expected, is one of the solid tumours where p53 is implicated. Before the age of five, soft tissue sarcomas, osteosarcoma, and early-onset breast cancer are among them. P53 has been linked to solid tumours including melanoma, lung, colon, bladder, breast, skin, oesophagus, liver, gastrointestinal tract, cervix, head and neck, ovaries, and melanoma.

This rearrangement can be found using FISH in cells that are in interphase or metaphase.