CSF For AFB Culture MGIT Test Cost & Procedure

Although CSF for AFB testing may be used to detect a variety of acid-fast bacilli, it is most frequently used to detect active tuberculosis (TB) infection caused by Mycobacterium tuberculosis, the most medically significant AFB.

AFB testing can identify a variety of acid-fast bacilli. However, it is most frequently used to spot active tuberculosis (TB) infections brought on by Mycobacterium tuberculosis, the most significant AFB from a medical standpoint.

Describe CSF

All vertebrates have this clear, colourless bodily fluid, which is found in the tissue that covers their brains and spinal cords. Any bilateral animals' bodily fluid detected outside the cells is replaced.

Why is this test recommended?

To aid in diagnosing tuberculosis (TB) and other Mycobacterium species infections, often known as acid-fast bacilli (AFB), in those at risk of contracting mycobacterial illnesses; to track the efficacy of treatment.

When to Be Tested?

• If you exhibit lung infection symptoms such as a persistent cough, weight loss, fever, chills, or weakness, that could indicate TB or a nontuberculous mycobacterial (NTM) infection.
• If you fall into a high-risk category for developing active TB and have a positive IGRA blood test or Tuberculin skin test (TST)
• While you are receiving treatment for TB when you have a skin infection or another body site illness that mycobacteria may cause.

Test's Objective (Indications)

•  This unique stain is used to identify Mycobacterium tuberculosis in various specimens, including tissue, caseous material, and sputum.
• The AFB smear stain is used to track the progress of tuberculosis treatment.
• The patient: should have an AFB stain on their sputum.
  • cough
  • Sweating at night.
  • Anorexia
  •  Fever.
  • Hemoptysis
  • Unaccounted-for weight loss.
  • Weakness and exhaustion.
  • A chest ache.

Recommend AFB stain in high-risk patients such as those who are immunocompromised, are alcoholics or have recently come into contact with TB patients.

Procedure

1. Make a smear of the sample culture and heat fix it over blue heat on a clean, sterile microscopic slide.

2. Pour and flood the smear with carbon Fuschia, then heat it slowly until fumes appear.

3. Let it sit for five minutes, then rinse it off with gently running water from the faucet.

4. Add 20% sulfuric acid and wait a minute or two. Continue doing this until the smear turns pink.

5. Use water to rinse the acid off.

6. Cover the stain with methylene blue dye, let it sit for a few minutes, and then rinse it with water.

7. Let the stain air dry before checking it with an oil immersion lens.

Results:

Positive:

The acid-fast bacilli are red colour rods, and the background is blue. This is a positive result.

2. When these Rods are seen, they indicate active tuberculosis.

3. The culture may confirm a positive AFB stain.

The unfavourable outcome is:

A negative result indicates that: 1. There is no infection.

2. There weren't enough bacteria to be seen under a microscope.

3. There may be additional causes for the symptoms.