Fungus Direct Examination By Fluorescence - Book Test at Low Cost

Direct examination of fungi using fluorescence is a technique used to detect and identify fungal infections in clinical samples. The process involves the use of a fluorescent stain, such as calcofluor white or acridine orange, which binds to the fungal cell wall or DNA, respectively, causing the fungi to fluoresce under a UV light.

Fungal direct examination by fluorescence is a rapid and reliable method for identifying fungi in clinical samples. It can be used to diagnose a variety of fungal infections, including dermatophytosis, candidiasis, aspergillosis, and cryptococcosis.

Why is Fungus Direct Examination by Fluorescence done?

Fungus direct examination by fluorescence is done for several reasons, including:

Diagnosis of fungal infections: One of the primary reasons for performing a fungal direct examination by fluorescence is to diagnose fungal infections. This technique is particularly useful for identifying fungal species that are difficult to culture or grow in the laboratory, such as certain types of dermatophytes or Candida species.

Rapid results : Direct examination by fluorescence can provide results in a matter of minutes, which can be important in cases where a rapid diagnosis is necessary to initiate prompt treatment.

Monitoring treatment response: Direct examination by fluorescence can also be used to monitor the response of fungal infections to antifungal therapy. The disappearance of fungal structures under fluorescence microscopy can indicate successful treatment.

Overall, fungal direct examination by fluorescence is an important diagnostic tool in the management of fungal infections, particularly in cases where rapid diagnosis is essential for prompt treatment.

Sample for Fungus Direct Examination by Fluorescence

A variety of clinical samples can be used for fungus direct examination by fluorescence, depending on the suspected site of infection. Some common samples include:

• Skin scrapings : Used to diagnose superficial fungal infections of the skin, hair, and nails, such as dermatophytosis or onychomycosis.
• Sputum : Used to diagnose fungal infections of the respiratory tract, such as aspergillosis or pneumocystis pneumonia.
• Cerebrospinal fluid (CSF) : Used to diagnose fungal infections of the central nervous system, such as cryptococcosis.
• Blood : Used to diagnose invasive fungal infections, such as candidemia or invasive aspergillosis.
• Body fluids : Other body fluids, such as urine or pleural fluid, can also be used to diagnose fungal infections depending on the site of infection.

Procedure

The steps involved in performing a fungal direct examination by fluorescence include:

Collecting a clinical sample : The clinical sample can be obtained from various sources, such as skin scrapings, nail clippings, sputum, or blood.

Preparing the sample : Depending on the type of sample, it may need to be prepared differently. For example, skin scraping may need to be treated with potassium hydroxide (KOH) to dissolve skin cells and expose the fungi. A sputum sample may need to be processed to remove mucus and debris.

Staining the sample: A fluorescent stain, such as calcofluor white or acridine orange, is added to the sample and allowed to incubate for a specific period.

Examining the sample under UV light : The stained sample is then viewed under a fluorescence microscope, which emits UV light to excite the fluorescent molecules and reveal the fungal structures.

Cost of Fungus Direct Examination By Fluorescence

The cost of Fungus Direct Examination By Fluorescence near me in Delhi ranges from INR 200 to INR 1500