HIV RNA PCR Qualitative Test Cost & Procedure

Assistance in the treatment of patients with HIV-1 infection is the qualitative HIV-1 RNA PCR test. It should be mentioned that this test is used to understand the patient's prognosis and track the effects of antiretroviral drug therapy, not for initial diagnosis or even for confirmation of HIV-1 infection.

Why was this test suggested?

• Real-time PCR is a molecular biology laboratory technique based on the polymerase chain reaction (PCR).
• Instead of at the end, like in the conventional PCR, it monitors the amplification of a specific DNA molecule while it is happening, or in real-time.
• Quantitative or qPCR, also known as real-time PCR.

Preparation

Specimen Required

Gather Plasma Preparation Tube, Pink (K2EDTA), or Lavender (EDTA) (PPT). Prepare the specimens: 24 hours after collecting, remove them from cells. 3 mL of plasma should be transferred to an ARUP Standard Transport Tube and frozen. (Min: 1.5 mL) Storage/Transport Frozen as the temperature. Unacceptable circumstances Serum. Heparinized samples. specimens submitted in a tube for making plasma. Stability: Following cell separation: 24 hours ambient, 6 days in a refrigerator, and 6 weeks in a freezer

Uses

RNA analysis, gene expression analysis study of genetic variation identification of pathogens HIV-1 infection in those with acute or early HIV diagnosis one infection HIV-1 infection in infants under the age of 18 months who were delivered to infected moms is diagnosed calculating the viral load (plasma HIV-1 RNA levels) in HIV-1-positive individuals

Procedure

• Before beginning anti-HIV-1 medication therapy. Real-time PCR can begin using a DNA template as the starting template.
• Enzymes called reverse transcriptases are typically derived from retroviruses that carry RNA.
• The real-time PCR is then supplemented with a tiny portion of the reverse transcription process.
• When oligo-dT primers or random oligomers are used for reverse transcription, multiple transcripts from a single RT reaction can be examined by PCR.
• This approach makes it possible to process numerous samples quickly and is simple to automate. Since fewer handling steps are necessary, samples can be reproduced accurately from one to the next, lowering the danger of contamination.

What is the Cost HIV RNA PCR Qualitative 

The average price for this HIV RNA PCR Qualitative  is seen to differ from one center to another. The price range is said to be between INR 2500 to INR 11500 in Delhi, India.