HLA DNA High Resolution Typing - C Locus Test and Its Price in India

It is a type of high resolution which is defined as a set of the allele that means a DNA sequence that specifies and converts in coded form the same protein sequence for the peptide binding region of an HLA molecule.

Use

This test is used for HLA typing at least four digits in bone marrow transplants.

HLA typing:

Typing of human leukocyte antigen. Some factors related to the immune system are associated with HLA typing. This test will help to identify the person to whom they can safely donate bone marrow, organs or cord blood to someone who needs to transplant.

Purpose of HLA typing 

The most crucial purpose of HLA typing is to identify the person who can provide the safest bone marrow or any organ for transplantation. . NGS-based HLA typing gives high-resolution matching, which can provide high-resolution, phase-defined HLA DNA sequences unambiguously and helps to avoid limitations compared to the traditional line-based Typing methods.

Methods of HLA typing antigen

There are mainly three methods of HLA typing antigen that are cellular, serological and DNA methods Method of DNA extraction. Firstly, lab experts dubbed the site of the patient with antiseptic. After that, venous blood of about 2 ml was collected from the patient's veins using an EDTA anticoagulant tube. From peripheral blood, genomic DNA is removed and stored at low temperatures.

Results of the HLA typing ( DNA extraction):

The potential donor sample is compared with the recipient and will decide the typing result. Results show how many antigens are matched to the donor and how many antigens match the donor. If there are zero mismatches, it will indicate that the organ donated by the donor to the recipient is safe to transplant an organ or bone marrow or any cord blood, and there is significantly less chance of being rejected by the recipient.