MLH1 Gene Sequencing Test Cost & Procedure

The MLH1 gene produces instructions for a protein that is necessary for DNA repair. It assists to fix errors created during DNA replication. The mismatch repair gene family includes the MLH1 gene.

What Is MLH1 Gene Sequencing?

The genetic material in the MLH-1 gene is sequenced using MLH1 gene analysis. DNA repair protein is encoded by the MLH1 gene. The test is used to identify medical conditions such as ovarian cancer, Lynch syndrome, and Constitutional mismatch repair failure syndrome.

Normal Function:

The scope of Normal Function has increased.

A protein that is crucial for mending DNA is made using instructions from the MLH1 gene. This protein helps to rectify errors made during DNA replication (DNA copying), which is a necessary step before cell division. The MLH1 protein and PMS2 protein (produced by the PMS2 gene) unite to form a two-protein complex known as a dimer. This complex coordinates the activity of other proteins that fix errors made during DNA replication. A portion of defective DNA is removed and replaced with a stretch of intact DNA to complete the repairs. The mismatch repair (MMR) genes are a group of genes that includes the MLH1 gene.

MLH1 Gene Sequencing Test Preparation:

• Your vein is punctured, and a sterile tube is used to collect 5 ml of your entire blood.
• Because specimen stability is crucial, blood is stored at room temperature.
• The blood sample should not be frozen, it is urged.
• The blood sample may be kept for 8 days at room temperature or in the refrigerator, but freezing the sample is not permitted.

MLH1 Gene Sequencing Test Applications:

• Patients with colorectal cancer may benefit from MLH1 gene sequencing, particularly if their immunohistochemistry and microinstability results reveal a decline of MLH1 protein staining.
•  It is also used to evaluate mismatch repair genes and find germline mutations.
• This detection helps with early intervention or prevention.

MLH1 Gene Sequencing Test Procedure:

The two key techniques utilized in the MLH1 gene sequencing test are polymerase chain reaction and dye terminator sequencing reaction.