Through the study of macromolecules like proteins and nucleic acids, which are vital for life, molecular biology helps doctors better comprehend a patient's biological makeup. A patient's susceptibility to disease and potential reactions to a helpful treatment alternative can be ascertained by learning about their genetic variations.
The knowledgeable staff of Ganesh Diagnostic and Imaging Centre maintains current on the most recent developments in molecular biology testing. Focus on patient-specific diagnostic data that encourages efficient decision-making and enhances patient care while cost-effectively reducing it.
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The PCR technique is widely used and recognized in the molecular biology department. It is used to make multiple copies or the amplification of the small sections of your DNA or the gene. Using the PCR technique, it is quite possible to produce thousands to even make a millions of copies in the particular section of the DNA from very minute and tiny amounts of DNA. The PCR is known to be a common tool which is used in your medical and in your biological research labs.
As we have seen, that the DNA is microscopic in nature, there would be lots of copies generated until there can be detection of that sample by the naked eye. Hence, the PCR is considered as an important tool: it starts producing enough copies of your DNA sequence, that could either be seen or even manipulated in that region of the DNA.
The results that you can come across with respect to the RT-PCR reports, is that the proposed criteria (based on scientific evidence) is that it presents with a 98.5% (95% confidence interval [95% CI] 97.5–99.5%)and sensitivity, 70% (95% CI 65.8–74.2%) is the specificity, 85.5% (95% CI 83.4–87.7%) for its accuracy. The PPV of around 79.7% (95% CI 76.6–82.7%) and the NPV is presented with 97.6% (95% CI 95.9–99.2%).
In many instances, the At-home tests or your antigen tests conducted at our diagnostic center has the ability to give the results in about 15-30 minutes. The PCR long with other molecular tests would be sent to our lab, and in a period of 1-2 days after the lab would get the test result.
The Real-time PCR is considered to be a highly sensitive method, which is not only for its detection, but also for the characterization of any minimal (minute) residual disease collected from the blood samples of the patients with epithelial cancer. Moreover, the PCR is also known to detect and establish an early diagnosis of breast cancer.
For establishing another testing method for qPCR, it can be applied for detection of the viral causes of your cancer like the mouse mammary tumor virus, in case of bovine leukemia virus, the Human Papillomavirus and the Epstein-Barr virus.