Stain: Grocott Methenamine Silver (GMS) Test Cost and Procedure

The Grocott methenamine silver (GMS) stain is frequently used to identify fungi on tissue slices and cytosmears. It gives the fungal profiles a black tint and the background a light green hue. Both pathogenic and nonpathogenic fungi, as well as melanin, are stained.

What is the purpose of GMS stain?

The Grocott methenamine silver (GMS) stain is frequently used to identify fungi on tissue slices and cytosmears. It gives the fungal profiles a black tint and the background a light green hue. Both pathogenic and nonpathogenic fungi, as well as melanin, are stained.

What is the GMS stain's history?

A histology stain called Grocott-Methenamine Gomori's Silver (GMS) color primarily identifies carbohydrates in fungal bacteria. György Gömöri, a physician from Hungary who created the staining approach, is honored by having his staining method named after him.

Which dye is applied to fungi?

Solutions of Hexamine Silver with Schiff's Reagent

The Grocott's silver (GMS) stain is arguably the most used fungal stain. However, even the most seasoned histology professionals may have difficulties due to the somewhat unpredictable nature of silver staining.

What are the three fungus stains?

Fungal staining's fundamental principle

Periodic acid Schiff (PAS), Lactophenol Cotton Blue (LPCB), and Periodic acid Schiff and Gomari Methenamine Silver (PAS and GMS) methods.

Which four varieties of fungi are there?

Based on how the fungus reproduces sexually,

It is classified into a division.

• Clinical traits
• GMS stain cannot identify an organism with certainty.

Other techniques are preferred for identification, Culture (which can also acquire susceptibility data) (which can also gather susceptibility data). Alternative methods of assessing non-tissue samples (such as PCR, blood culture, galactomannan, -D-glucan, antibody detection, etc.)

Molecular analysis

For assessing formalin-fixed, paraffin-embedded (FFPE) blocks, there aren't many clinically recognized assays available.

Formalin crosslinking causes numerous false negatives. On a few formalin-fixed, paraffin-embedded (FFPE) blocks, extra unique stains.

Which two stains can be used to identify fungi?

The GMS stain has a signal-to-noise problem since it also stains tissue reticulin and inflammatory cells (lysosomes), in addition to fungus. However, it is more sensitive than the PAS stain.

Are fungus Gram positive stains?

In a direct smear, fungi's tubular filaments, known as hyphae, may also be visible. Hyphae can branch or entwine and stain Gra-positive. Although it is less sensitive than the specific stains used for parasites, the Gram stain candetect them.

What is the Cost of Stain : Grocott Methenamine Silver (GMS) Test 

The Stain : Grocott Methenamine Silver Test cost varies by region and facility. So, constantly get tested at trustworthy diagnostic centers like Ganesh Diagnostic. It is staffed by trained and experienced professionals to assure patient and visitor safety.

The average price for this test is seen to differ from one center to another. The price range is said to be between INR 1000 to INR 2500 in Delhi, India.