Stain, Periodic Acid-Schiff (PAS)

Q: What are the PAS stains frequently used in the diagnosis of?

Periodic acid-Schiff (PAS) staining is commonly used in histology laboratories to detect glycogen deposits, particularly in the liver. It can also reveal glycogen granules in tumors of various organs such as bladder, kidney, ovary, pancreas, and lung.

Q: What stains positive with your PAS method?

Glycogen stains positive with the PAS method. Glycogen shows a PAS-positive, PASD-negative staining pattern, while neutral mucosubstances exhibit a double positive pattern.

Q: What stands behind the principle of PAS staining?

PAS staining detects aldehyde groups formed by the prior oxidation of carbohydrates. It is commonly used to evidence the presence of these groups in histological samples.

Q: What is the Color of periodic acid Schiff stain?

Schiff's reagent rapidly turns into a red-purple color when reacting with glycogen. A deteriorated Schiff's reagent may produce a deep blue-purple color with delayed reaction.

Q: What are the limitations of PAS stain?

The PAS stain may not visualize fungal species or non-viable organisms as effectively as other stains like Gomori's Methenamine Silver (GMS).

Q: Is hematoxylin used in PAS stain?

For PAS staining, Harris Hematoxylin Histology Stain is used.

Q: Where can I get myself tested for Periodic Acid-Schiff (PAS)?

You can get yourself tested for Periodic Acid-Schiff (PAS) at Ganesh Diagnostic and Imaging Center. Trained staff and technicians will assist you in getting your sample collected.

Book Stain, Periodic Acid-Schiff (PAS) Appointment Online Near me at the best price in Delhi/NCR from Ganesh Diagnostic. NABL & NABH Accredited Diagnostic centre and Pathology lab in Delhi offering a wide range of Radiology & Pathology tests. Get Free Ambulance & Free Home Sample collection. 24X7 Hour Open. Call Now at 011-47-444-444 to Book your Stain, Periodic Acid-Schiff (PAS) at 50% Discount.

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Description
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FAQ

The Periodic Acid-Schiff Dye is a widely utilized strategy in histopathology for the indication of glucose and glucose-rich mixtures in tissues. PAS dyes demonstrate polysaccharides, mucin, glycogen, specific glycoproteins and glycolipids, basement membrane, and specific fungus in tissues.

Significance and Uses of Periodic Acid-Schiff Stain

PAS enables the indication of glycogen, cellulose, and starch. This is valuable to see glycogen residues in the liver when glycogen repository ailment is presumed.
The basement membrane of varied tissues may also be imagined through the PAS pigment. The PAS is normally utilized to indicate the consistency of the glomerular basement membrane when the renal ailment is being evaluated.
Mucin, especially acid mucin, is indicated by PAS. It is crucial for endocervical glands, intestinal glands, and bronchial glands.
PAS enables the indication of cerebrosides and gangliosides. It assists in the detection of Gaucher’s disease, Krabbe’s ailment and lysosomal warehouse ailments, etc.
Specific dyes such as lipofuscin and tints of Dubin-Johnson syndrome are demonstrated by PAS pigment.
Russell bodies of plasma compartments are smudged by PAS.

The precept of PAS Stain

The Glycol class of carbohydrates is corroded by occasional acids to discharge dialdehydes. These dialdehydes in subsequent mixture with Schiff’s reagent outcome in the appearance of magenta colored intricate, localized at the area of aldehyde impression.

Preparation of Reagents for PAS Stain

Periodic Acid Solution

The occasional acid solution, utilized for oxidation is utilized in any resilience between 0.49% and 2.49%. One percent solution is selected.

>> Occasional acid= one gm
>> Distilled water= Hundred ml

Schiff’s Reagent

>> Fuchsin Basic: 0.9 gm
>> Purified water: 100 ml
>> Sodium metabisulphite: 1.9 gm
>> Conc. HCl: 1.9 ml
>> Charcoal activated: 0.29 gm

The technique of PAS Stain

Deparaffinization: heat the slip on the flame and spot it in the xylene. Reiterate the antidote to peel off the wax.
Hydration: Drain xylene and hydrate the tissue compartment by enacting lessening the attention of liquor baths and water.
Oxidation: Place the compartments in the periodic acid solution for five-ten minutes.
Clean: Wash in at least two modifications of refined water.
Antidote with Schiff’s reagent: Cover with Schiff’s reagent for an half hour.
Rinse: Rinse in operating tap moisture for five-ten minutes.
Counterstain: sleeve with hematoxylin for three-five minutes. Distinguish and blue for the compartments.
Dehydration: Dehydrate in boosting the concentration of liquor.
Clearing: Put glides in two xylene baths for clemency.
Mounting: Charger in DPX or other mounting media.
Scrutinize under a microscope.

Outcomes and Interpretation

The substances favorable for PAS are

Polysaccharides: Glycogen, cellulose, and starch. Many leukocytes comprise glycogen, capsules of fungi, actinomycosis, and bacteria.

Test Type	Stain, Periodic Acid-Schiff (PAS)
Includes	Stain, Periodic Acid-Schiff (PAS) Test (Hematology)
Preparation
Reporting	Within 24 hours*
Test Price	₹ 400 ~~₹ 800~~

Frequently Asked Questions

What are the PAS stains frequently used in the diagnosis of?

It is seen that periodic acid-Schiff (PAS) staining procedure could be considered most commonly being used in your histology laboratory to further detect your glycogen deposits in liver. This is where the glycogen storage disease could be suspected. Moreover, the Glycogen granules would be visible in the tumors of the following:

Bladder
Kidney
Ovary
Pancreas
Lung

What stains positive with your PAS method?

In general, it is seen that the glycogen could be digested by the enzyme diastase whereas your neutral mucosubstances cannot do the same. Hence, your glycogen shows the PAS-positive whereas PASD-negative staining pattern while the neutral mucosubstances could have the double positive pattern.

What stands behind the principle of PAS staining?

The PAS staining (Periodic Acid-Schiff) is said to be one of the most commonly used stains in the histology department and it is also used to further evidence presence of an aldehyde groups which gets formed by your prior test with oxidation of any carbohydrates.

What is the Color of periodic acid Schiff stain?

Your Good Schiff's reagent could have ability to the rapidly turn into a red purple color. When there is a deteriorating with Schiff's reagent will with any delayed reaction with color produced would be a deep blue-purple.

What are the limitations of PAS stain?

The drawback and limitation of the PAS stain with your comparison to any GMS is which would not visualize any of the fungal species well, and the non-viable organisms which usually are not that well stained.

Is hematoxylin used in PAS stain?

For your PAS (Periodic Acid-Schiff): The Harris Hematoxylin Histology Stain is used.
When it is designed for only the research use, it would not be approved for the human or the animal use, along with clinical diagnosis.

Where can I get myself tested for Periodic Acid-Schiff (PAS)?

You could get yourself tested for Periodic Acid-Schiff (PAS) at Ganesh Diagnostic and Imaging Center. We would be happy to assist you in getting your sample by trained staff and technicians.

Can I get doctors consultation at Ganesh Diagnostic for Hemophilia Panel?

Yes, a free doctor’s consultation is available for all individuals. Either before the test, or after the test, you can avail this facility at our center, online or in person.

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