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The Periodic Acid-Schiff Dye is a widely utilized strategy in histopathology for the indication of glucose and glucose-rich mixtures in tissues. PAS dyes demonstrate polysaccharides, mucin, glycogen, specific glycoproteins and glycolipids, basement membrane, and specific fungus in tissues.
The Glycol class of carbohydrates is corroded by occasional acids to discharge dialdehydes. These dialdehydes in subsequent mixture with Schiff’s reagent outcome in the appearance of magenta colored intricate, localized at the area of aldehyde impression.
The occasional acid solution, utilized for oxidation is utilized in any resilience between 0.49% and 2.49%. One percent solution is selected.
>> Occasional acid= one gm
>> Distilled water= Hundred ml
>> Fuchsin Basic: 0.9 gm
>> Purified water: 100 ml
>> Sodium metabisulphite: 1.9 gm
>> Conc. HCl: 1.9 ml
>> Charcoal activated: 0.29 gm
The substances favorable for PAS are
Polysaccharides: Glycogen, cellulose, and starch. Many leukocytes comprise glycogen, capsules of fungi, actinomycosis, and bacteria.
Test Type | Stain, Periodic Acid-Schiff (PAS) |
Includes | Stain, Periodic Acid-Schiff (PAS) Test (Hematology) |
Preparation | |
Reporting | Within 24 hours* |
Test Price |
₹ 400
|
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