Stain, Periodic Acid-Schiff (PAS) Test Cost & Procedure

The Periodic Acid-Schiff  Dye is a widely utilized strategy in histopathology for the indication of glucose and glucose-rich mixtures in tissues. PAS dyes demonstrate polysaccharides, mucin, glycogen, specific glycoproteins and glycolipids, basement membrane, and specific fungus in tissues.

Significance and Uses of Periodic Acid-Schiff  Stain

• PAS enables the indication of glycogen, cellulose, and starch. This is valuable to see glycogen residues in the liver when glycogen repository ailment is presumed.
• The basement membrane of varied tissues may also be imagined through the PAS pigment. The PAS is normally utilized to indicate the consistency of the glomerular basement membrane when the renal ailment is being evaluated.
• Mucin, especially acid mucin, is indicated by PAS. It is crucial for endocervical glands, intestinal glands, and bronchial glands.
• PAS enables the indication of cerebrosides and gangliosides. It assists in the detection of Gaucher’s disease, Krabbe’s ailment and lysosomal warehouse ailments, etc.
• Specific dyes such as lipofuscin and tints of Dubin-Johnson syndrome are demonstrated by PAS pigment.
• Russell bodies of plasma compartments are smudged by PAS.

The precept of PAS Stain

The Glycol class of carbohydrates is corroded by occasional acids to discharge dialdehydes. These dialdehydes in subsequent mixture with Schiff’s reagent outcome in the appearance of magenta colored intricate, localized at the area of aldehyde impression.

Preparation of Reagents for PAS Stain

Periodic Acid Solution

The occasional acid solution, utilized for oxidation is utilized in any resilience between 0.49% and 2.49%.  One percent solution is selected.

>> Occasional acid= one gm
>> Distilled water= Hundred ml

Schiff’s Reagent

>> Fuchsin Basic: 0.9 gm
>> Purified water: 100 ml
>> Sodium metabisulphite: 1.9 gm
>> Conc. HCl: 1.9 ml
>> Charcoal activated: 0.29 gm

The technique of PAS Stain

1. Deparaffinization: heat the slip on the flame and spot it in the xylene. Reiterate the antidote to peel off the wax.
2. Hydration: Drain xylene and hydrate the tissue compartment by enacting lessening the attention of liquor baths and water.
3. Oxidation: Place the compartments in the periodic acid solution for five-ten minutes.
4. Clean: Wash in at least two modifications of refined water.
5. Antidote with Schiff’s reagent: Cover with Schiff’s reagent for an half hour.
6. Rinse: Rinse in operating tap moisture for five-ten minutes.
7. Counterstain: sleeve with hematoxylin for three-five minutes. Distinguish and blue for the compartments.
8. Dehydration: Dehydrate in boosting the concentration of liquor.
9. Clearing: Put glides in two xylene baths for clemency.
10. Mounting: Charger in DPX or other mounting media.
11. Scrutinize under a microscope.

Outcomes and Interpretation

The substances favorable for PAS are

Polysaccharides: Glycogen, cellulose, and starch. Many leukocytes comprise glycogen, capsules of fungi, actinomycosis, and bacteria.