Bronchial Lavage for AFB Stain Test Cost & Procedure

 However, the most frequent application of bronchial washing for AFB testing is to detect active tuberculosis (TB) infection brought on by Mycobacterium tuberculosis, the most significant AFB from a medical standpoint. AFB testing can identify a variety of acid-fast bacilli. However, it is most frequently used to spot active tuberculosis (TB) infections brought on by Mycobacterium tuberculosis, the most significant AFB from a medical standpoint.

Why Take a Test?

When to Be Tested:

• If you exhibit lung infection symptoms such as a persistent cough, weight loss, fever, chills, or weakness, that could indicate TB or a nontuberculous mycobacterial (NTM) infection.

• If you fall into a high-risk category for developing active TB and have a positive IGRA blood test or Tuberculin skin test (TST)

• When you have a possible mycobacterial infection of the skin or another body location; when you are receiving TB therapy; when you have a positive IGRA Dialog test or Tuberculin skin test, you are in a high-risk group for developing active TB.

Test's Objective (Indications):

1. This unique stain is used to identify Mycobacterium tuberculosis in various specimens, including tissue, caseous material, and sputum.

2. The AFB smear stain, which is used to track the progress of tuberculosis treatment.

3. The patient: They should have an AFB stain on their sputum. One cough

•  Sweating at night
• Anorexia
• Fever
• Hemoptysis
• Unaccounted-for weight loss
• Our weakness and exhaustion
•  A chest ache

4. Recommend AFB stain in high-risk patients such as those who are immunocompromised, are alcoholics or have recently come into contact with TB patients.

Collection of Specimens and Instructions

Kind of Specimen: Spinal fluid, pericardial, peritoneal, pleural, and other body fluids

Sterile container or tube Collection Source identification is required.

Add two patient identifiers to the label.

Procedure:

1. Create a smear of the sample culture and heat fix it over blue heat on a clean, sterile microscope slide.

2. Pour and flood the smear with carbon Fuschia, then heat it slowly until fumes appear.

3. Let it sit for five minutes, then rinse it off with

gently running water from the faucet.

4. Add 20% sulfuric acid and wait a minute or two. Continue doing this until the smear turns pink.

5. Use water to rinse the acid off.

6. Pour methylene blue dye all over the smear and let it sit for a few minutes. 7. Let the stain air dry before checking it with an oil immersion lens.

Positive results include:

1. The red colour rods of the acid-fast bacilli contrast with the blue background.

2. The presence of these rods signifies active tuberculosis.

3. The culture may verify that the AFB stain is positive.

Negative:

1. There weren't enough bacteria to be visible under a microscope

2. There may be additional causes for the symptoms.